Publications

Nicacio, K. J., Ióca, L. P., Fróes, A. M., Leomil, L., Appolinario, L. R., Thompson, C. C., Thompson, F. L., Ferreira, A. G., Williams, D. E., Andersen, R. J., Eustáquio, A. S., Berlinck, R. G. S. (2017). Cultures of the Marine Bacterium Pseudovibrio denitrificans Ab134 Produce Bromotyrosine-Derived Alkaloids Previously Only Isolated from Marine Sponges. J. Nat. Prod. 80(2), 235-240. DOI: 10.1021/acs.jnatprod.6b00838 (Link)

Agarwal V, Miles ZD, Winter JM§, Eustáquio AS, El Gamal AA, and Moore BS*⊥ (2017) Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse. Chem Rev  117 (8), 5619–5674. DOI: 10.1021/acs.chemrev.6b00571 (Link)

Eustáquio AS*, Chang LP, Steele GS, O’Donnell CJ, Koehn FE (2016) Biosynthetic engineering and fermentation media development leads to gram-scale production of spliceostatin natural products in Burkholderia sp. Metab Eng 33, 67-75 (Link).

Highlighted in: Gene Tweaking Boosts Spliceostatin Synthesis (2016) C&E News 94, 21.

Eustáquio AS*, Ziemert N*. (2016) Identification of natural product biosynthetic gene clusters from bacterial genomic data. Chemical Biology Informatics and Modeling, Methods in Pharmacology and Toxicology Springer Protocol Series, book chapter in press.

Eustáquio AS*, Janso JE, Ratnayake AS, O’Donnell CJ, Koehn FE (2014) Spliceostatin hemiketal biosynthesis in Burkholderia spp. is catalyzed by an iron/α-ketoglutarate-dependent dioxygenase. Proc Natl Acad Sci USA 111, E3376–E3385 (Open Access Link)

Highlighted in: Chemists Stabilize Spliceostatins (2014) C&E News 92, 34.

Janso JE, Haltli BA, Eustáquio AS, Kulowski K, Waldman AJ, Zha L, Nakamura H, Bernan VS, He H, Carter GT, Koehn FE, Balskus EP (2014) Discovery of the lomaiviticin biosynthetic gene cluster in Salinispora pacifica. Tetrahedron 70, 4156-4164.

Jones AC, Ottilie S, Eustáquio AS, Edwards DJ, Gerwick L, Moore BS, Gerwick WH (2012) Evaluation of Streptomyces coelicolor A3(2) as a heterologous expression host for the cyanobacterial protein kinase C activator lyngbyatoxin A. FEBS J 279, 1243-51.

Lechner A, Eustáquio AS, Gulder TA, Hafner M, Moore BS (2011) Selective overproduction of the proteasome inhibitor salinosporamide A via precursor pathway regulation. Chem Biol 18, 1527-36.

Highlighted in:     Better chemistry through regulation (2011) Chem Biol 18, 1515-1516.

Miyanaga A, Janso JE, McDonald L, He M, Liu H, Barbieri L, Eustáquio AS, Fielding EN, Carter GT, Jensen PR, Feng X, Leighton M, Koehn FE, Moore BS (2011) Discovery and assembly-line biosynthesis of the lymphostin pyrroloquinoline alkaloid family of mTOR inhibitors in Salinispora bacteria. J Am Chem Soc 133, 13311-3.

Udwary DW, Gontang EA, Jones AC, Jones CS, Schultz AW, Winter JM, Yang JY, Beauchemin N, Capson TL, Clark BR, Esquenazi E, Eustáquio AS, Freel K, Gerwick L, Gerwick WH, Gonzalez D, Liu WT, Malloy KL, Maloney KN, Nett M, Nunnery JK, Penn K, Prieto-Davo A, Simmons TL, Weitz S, Wilson MC, Tisa LS, Dorrestein PC, Moore BS (2011) Significant natural product biosynthetic potential of actinorhizal symbionts of the genus Frankia, as revealed by comparative genomic and proteomic analyses. Appl Environ Microbiol 77, 3617-25.

Eustáquio AS, Nam SJ, Penn K, Lechner A, Wilson MC, Fenical W, Jensen PR, Moore BS (2011) The discovery of salinosporamide K from the marine bacterium “Salinispora pacifica” by genome mining gives insight into pathway evolution. Chembiochem 12, 61-4.

Eustáquio AS, O’Hagan D and Moore BS (2010) Engineering fluorometabolite production: fluorinase expression in Salinispora tropica yields fluorosalinosporamide. J Nat Prod 73, 378-382.

Highlighted in: Fluorinase success. Biosynthesis: In a chemical first, an engineered microbe yields a bioactive fluorinated compound (2010) C&E News 88, 7.

Deng H, McMahon SA, Eustáquio AS, Moore BS, Naismith JH and O’Hagan D (2009) Mechanistic insights into water activation in SAM hydroxide adenosyltransferase (duf-62). Chembiochem 10, 2455-2459.

Liu Y, Hazzard C, Eustáquio AS, Reynolds KA and Moore BS (2009) Biosynthesis of salinosporamides from a,b-unsaturated fatty acids: implications for extending polyketide synthase diversity. J Am Chem Soc 131, 10376-10377.

Eustáquio AS, McGlinchey RP, Liu Y, Hazzard C, Beer LL, Florova G, Alhamadsheh MM, Lechner A, Kale AJ, Kobayashi Y, Reynolds KA and Moore BS (2009) Biosynthesis of the salinosporamide A polyketide synthase substrate chloroethylmalonyl-CoA from S-adenosyl-L-methionine. Proc Natl Acad Sci USA 106, 12295-12300.

Highlighted in: Better biosynthesis building blocks (2009) C&E News 87, 46.

Eustáquio AS, Härle J, Noel JP and Moore BS (2008) S-adenosyl-L-methionine hydrolase (adenosine-forming), a conserved bacterial and archeal protein related to SAM-dependent halogenases. Chembiochem 9, 2215-2219.

Eustáquio AS and Moore BS (2008) Mutasynthesis of fluorosalinosporamide, a potent and reversible inhibitor of the proteasome. Angew Chem Int Ed 47, 3936-3938.

Highlighted in: This week in therapeutics. 20S proteasome. (2008). SciBX 1, 11.

Eustáquio AS, Pojer F, Noel JP and Moore BS (2008) Discovery and characterization of a marine bacterial SAM-dependent chlorinase. Nat Chem Biol 4, 69-74.

Highlights of this paper include:

A key enzyme to the potency of an anticancer agent (2008) ALSNews 287.

Special Delivery (2007) Nature 450, 925.

Novel Biochlorination (2007) C&E News 85, 25.

Researchers Uncover Key Trigger for Potent Cancer-Fighting Marine Product UCSD News.

Moore BS, Eustáquio AS and McGlinchey RP (2008) Advances in and applications of proteasome inhibitors. Curr Opin Chem Biol 12, 434-440.

McGlinchey RP, Nett M, Eustáquio AS, Asolkar RN, Fenical W and Moore BS (2008) Engineered biosynthesis of antiprotealide and other unnatural salinosporamide proteasome inhibitors. J Am Chem Soc 130, 7822-7823.

Dangel V, Eustáquio AS, Gust B and Heide L (2008) novE and novG act as positive regulators of novobiocin biosynthesis. Arch Microbiol 190, 509-519.

Flatman RH, Eustáquio A, Li S-M, Heide L and Maxwell A (2006) Structure-activity relationships of aminocoumarin-type gyrase and topoisomerase IV inhibitors obtained by combinatorial biosynthesis. Antimicrob Agents Chemotherap 50, 1136-1142.

Eustáquio AS, Li S-M and Heide L (2005) NovG, a DNA-binding protein acting as a positive regulator of novobiocin biosynthesis. Microbiology 151, 1949-1961.

Eustáquio AS, Gust B, Galm U, Li S-M, Chater KF and Heide L (2005) Heterologous expression of novobiocin and clorobiocin biosynthetic gene clusters. Appl Environ Microbiol 71, 2452-2459.

Eustáquio AS, Gust B, Li S-M, Pelzer S, Wohlleben W, Chater KF and Heide L (2004) Production of 8’-halogenated and 8’-unsubstituted novobiocin derivatives in genetically engineered Streptomyces coelicolor strains. Chem Biol 11, 1561-1572.

Eustáquio AS, Luft T, Wang Z-X, Gust B, Chater KF, Li S-M and Heide L (2003) Novobiocin biosynthesis: inactivation of the putative regulatory gene novE and heterologous expression of genes involved in aminocoumarin ring formation. Arch Microbiol 180, 25-32.

Eustáquio AS, Gust B, Luft T, Li S-M, Chater KF and Heide L (2003) Clorobiocin biosynthesis in Streptomyces: identification of the halogenase and generation of structural analogs. Chem Biol 10, 279-288.